Gene Expression Data
Assay Details
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Assay
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Reference:
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J:113425
Suriben R, et al., Dact1 presomitic mesoderm expression oscillates in phase with Axin2 in the somitogenesis clock of mice. Dev Dyn. 2006 Nov;235(11):3177-83
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3690358
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Gene symbol:
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Dact1
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Gene name:
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dishevelled-binding antagonist of beta-catenin 1
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Probe:
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Dact1 cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Multiple probes were used to validate expression and all of them exhibited the same expression pattern. The current data is shown with probe Dact1 cDNA3, but it is possible that probes Dact1 cDNA1 (MGI:3690217) or Dact1 cDNA2 (MGI:3690218) were used.
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Results
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Specimen
1A:
embryonic day 9.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
1A
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Expression is at the rostral tip of the mesenchyme that will pinch off to form the next somite and it extends from the caudal tip rostrally up to the position where the second next somite will be.
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Specimen
1B:
embryonic day 9.5
(more )
Specimen
1B:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
1B
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Expression is at the rostral tip of the mesenchyme that will pinch off to form the next somite and it extends from the caudal tip rostrally up to the position where the second next somite will be.
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Specimen
1C:
embryonic day 9.5
(more )
Specimen
1C:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
1C
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Expression is at the rostral tip of the mesenchyme that will pinch off to form the next somite and it extends from the caudal tip rostrally up to the position where the second next somite will be.
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Specimen
1D:
embryonic day 9.5
(more )
Specimen
1D:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
1D
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Expression is at the rostral tip of the mesenchyme that will pinch off to form the next somite and it extends from the caudal tip rostrally up to the position where the second next somite will be.
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Specimen
1E:
embryonic day 9.5
(more )
Specimen
1E:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
1E
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Expression is at the rostral tip of the mesenchyme that will pinch off to form the next somite and it extends from the caudal tip rostrally up to the position where the second next somite will be.
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Specimen
1F:
embryonic day 9.5
(more )
Specimen
1F:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
1F
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Expression is at the rostral tip of the mesenchyme that will pinch off to form the next somite and it extends from the caudal tip rostrally up to the position where the second next somite will be.
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Specimen
2A:
embryonic day 9.5
(more )
Note: Double labeled: blue - Dact1; red - Axin2 (assay MGI:3690445).
Specimen
2A:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: blue - Dact1; red - Axin2 (assay MGI:3690445).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
2A
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Expression is at the rostral tip of the mesenchyme that will pinch off to form the next somite and it extends from the caudal tip rostrally up to the position where the second next somite will be.
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Specimen
2C:
embryonic day 9.5
(more )
Specimen
2C:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
2C
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Expression is at the rostral tip of the mesenchyme that will pinch off to form the next somite and it extends from the caudal tip rostrally up to the position where the second next somite will be.
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Specimen
2D:
embryonic day 9.5
(more )
Specimen
2D:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
2D Dact1
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Expression is at the rostral tip of the mesenchyme that will pinch off to form the next somite and it extends from the caudal tip rostrally up to the position where the second next somite will be.
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Specimen
2E:
embryonic day 9.5
(more )
Specimen
2E:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
2E Dact1
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Expression is at the rostral tip of the mesenchyme that will pinch off to form the next somite and it extends from the caudal tip rostrally up to the position where the second next somite will be.
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Specimen
2F:
embryonic day 9.5
(more )
Specimen
2F:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
2F Dact1
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Expression is at the rostral tip of the mesenchyme that will pinch off to form the next somite and it extends from the caudal tip rostrally up to the position where the second next somite will be.
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