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Gene Expression Data
Assay Details
Assay
Reference: J:112224 Maatouk DM, et al., DNA methylation is a primary mechanism for silencing postmigratory primordial germ cell genes in both germ cell and somatic cell lineages. Development. 2006 Sep;133(17):3411-8
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3665577
Gene symbol: Gcna
Gene name: germ cell nuclear acidic protein
Antibody: anti-GCNA
Detection system: Secondary antibody coupled to horseradish peroxidase
Results
Specimen 4A & Inset: embryonic day 12.5 (more )
Note: Double labeled: black - Gcna; red - Fut4 (assay MGI:3686606). Inset shows a higher magnification of double-labeled cells.
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 4A , 4A inset
Specimen 4B & Inset: embryonic day 8.5; Dnmt1tm2Enl/Dnmt1tm2Enl (more )
Note: Double labeled: black - Gcna; red - Fut4 (assay MGI:3686606). Inset shows a higher magnification of double-labeled cells.
Structure Level Pattern Image Note
TS13: primordial germ cell of yolk sac Present 4B , 4B inset Expression was detected in primordial germ cells in yolk sac endoderm.
Specimen 4C: embryonic day 9.5; Dnmt1tm2Enl/Dnmt1tm2Enl (more )
Note: Double labeled: black - Gcna; red - Fut4 (assay MGI:3686606).
Structure Level Pattern Image Note
TS15: primordial germ cell Present Regionally restricted 4C Expression was detected in primordial germ cells in the posterior region of the embryo.
Specimen 5A, 5B: embryonic day 9.5; Dnmt1tm1Jae/Dnmt1+ (more )
Structure Level Pattern Image Note
TS15: embryo Absent 5A , 5B
Specimen 5C, 5D: embryonic day 9.5; Dnmt1tm1Jae/Dnmt1tm1Jae (more )
Structure Level Pattern Image Note
TS15: embryo Present Patchy 5C , 5D Expression was detected in somatic cells scattered throughout the entire embryo.
Specimen 5E: embryonic day 9.5; Dnmt1tm2Enl/Dnmt1+ (more )
Structure Level Pattern Image Note
TS15: embryo Absent 5E
Specimen 5F, 5G: embryonic day 9.5; Dnmt1tm2Enl/Dnmt1tm2Enl (more )
Structure Level Pattern Image Note
TS15: embryo Present Patchy 5F , 5G Expression was detected in somatic cells scattered throughout the entire embryo.
Specimen 5H: embryonic day 8.5; Dnmt1tm2Enl/Dnmt1+ (more )
Structure Level Pattern Image Note
TS13: embryo Absent 5H
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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