Gene Expression Data
Assay Details
Assay
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Reference:
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J:98500
Meech R, et al., The homeobox transcription factor Barx2 regulates chondrogenesis during limb development. Development. 2005 May;132(9):2135-46
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3586389
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Gene symbol:
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Col2a1
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Gene name:
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collagen, type II, alpha 1
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Antibody:
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Abcam Clone 5B2.5
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
2A, D:
embryonic day 11.0
(more )
Note: Double labeled: green - Col2a1; red - Barx2 (assay MGI:3586388). D is a higher magnification of a portion of A.
Specimen
2A, D:
(close )
Genetic Background:
|
CD-1
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Age:
|
embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
|
Double labeled: green - Col2a1; red - Barx2 (assay MGI:3586388). D is a higher magnification of a portion of A.
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: limb mesenchyme |
Present |
Regionally restricted |
2A
,
2D
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Expression detected in the nuclei of the condensing pre-chondrogenic mesenchyme.
|
Specimen
2B, E:
embryonic day 12.5
(more )
Note: Double labeled: green - Col2a1; red - Barx2 (assay MGI:3586388). E is a higher magnification of a portion of B.
Specimen
2B, E:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
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Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Col2a1; red - Barx2 (assay MGI:3586388). E is a higher magnification of a portion of B.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: limb mesenchyme |
Trace |
Regionally restricted |
2B
,
2E
|
Expression detected in joint interzones.
|
TS20: limb digit |
Present |
Regionally restricted |
2E
,
2B
|
Expression detected in the digital rays.
|
Specimen
2C:
postnatal newborn
(more )
Note: Double labeled: green - Col2a1; red - Barx2 (assay MGI:3586388).
Specimen
2C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
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Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Col2a1; red - Barx2 (assay MGI:3586388).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: autopod joint |
Present |
Regionally restricted |
2C
|
Expression detected in the articular cartilage of carpal joints.
|
Specimen
2F:
postnatal newborn
(more )
Note: Double labeled: green - Col2a1; red - Barx2 (assay MGI:3586388).
Specimen
2F:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Col2a1; red - Barx2 (assay MGI:3586388).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: autopod joint |
Present |
Regionally restricted |
2F
|
Expression detected in the articular cartilage of phalangeal joints.
|
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