• hemangiogenesis in the normally avascular cornea is first seen as single sprouts around 2 weeks of age, by 3 weeks of age slit-lamp examination shows outgrowth of limbal blood vessels, and blood vessels cover the corneal surface by approximately 6 weeks of age. (J:98465)

• corneal hemangiogenesis precedes lymphangiogenesis by approximately one week, and there is no corneal blood vessel regression in the first year (J:98465)

• corneal hemangiogenesis and lymphangiogenesis can be inhibited by treatment with an antibody blocking vascular enothelial growht factor receptor 3 (J:98465)

• mice exhibit spontaneous corneal vascularization (J:114973)

• corneal injection of sflt-1 (secreted isoform of the cell surface receptor membrane-bound Flt1) protein reduces the area of vascularization compared to untreated mutants (J:114973)

• neovascularization is present in limbus at E18; vascularization grows and reaches center of cornea at 45 days (J:31798)

• focal areas ~1 mm in diameter are elevated above corneal surface (J:31798)

• epithelium has "dry", white appearance (J:31798)

• at 12 days of age, mice show mild edema of basal epithelial layers and corneal surface shows irregularities with focal areas ~1 mm in diameter elevated above corneal surface; corneal surface is increasingly roughened and irregular, until 70 days of age when irregularity stabilizes (J:31798)

• surface epithelial cell nuclei are pyknotic and cytoplasm has granular appearance (J:31798)

• eosinophilia is seen in superficial epithelial cells at 12 days of age, but is decreased by 60 days of age (J:31798)

• at 21 and 30 days of age, thickening of epithelium increases

• at 4 weeks of age the hyperplastic corneal epithelium has altered expression of keratin 14, involucrin, and lumican

• bilateral cataracts develop by 45-50 days of age; progressive lenticular opacification is observed to 8 months of age

• cataracts are manifested by liquefaction of lens fibers and posterior migration of lens epithelial nuclei

• in homozygotes, there are 10-fold more proliferating corneal epithelial cells than in wild-type or heterozygous mice

• in the cornea

• hemangiogenesis in the normally avascular cornea is first seen as single sprouts around 2 weeks of age, by 3 weeks of age slit-lamp examination shows outgrowth of limbal blood vessels, and blood vessels cover the corneal surface by approximately 6 weeks of age. (J:98465)

• corneal hemangiogenesis precedes lymphangiogenesis by approximately one week, and there is no corneal blood vessel regression in the first year (J:98465)

• corneal hemangiogenesis and lymphangiogenesis can be inhibited by treatment with an antibody blocking vascular enothelial growht factor receptor 3 (J:98465)

• mice exhibit spontaneous corneal vascularization (J:114973)

• corneal injection of sflt-1 (secreted isoform of the cell surface receptor membrane-bound Flt1) protein reduces the area of vascularization compared to untreated mutants (J:114973)

• neovascularization is present in limbus at E18; vascularization grows and reaches center of cornea at 45 days (J:31798)

• the aqueous humor has a normal protein and leukocyte count indicating that the corneal neovascularization and lymphangiogenesis are not mediated primarily by inflammation

• Corneal lymphangiogenesis begins with small sprouts around 3 weeks of age, a week after corneal hemangiogenesis begins, and lymphatic vessels are clearly visible by 4 to 6 weeks of age. Vessel fragmentation is seen as early as 2 months of age. Thus, corneal lymphangiogenesis is less severe than hemangiogenesis and comparatively delayed.

• This corneal lymphangiogenesis occurs in a low inflammatory context in which there are only slightly more CD45 positive cells at 4 weeks of age than in controls.