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Gene Expression Data
Assay Details
Assay
Reference: J:73506 Lowe LA, et al., Genetic dissection of nodal function in patterning the mouse embryo. Development. 2001 May;128(10):1831-43
Assay type: RNA in situ
MGI Accession ID: MGI:2159439
Gene symbol: Lefty1
Gene name: left right determination factor 1
Probe: Lefty1 probe3
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: Heterozygote Nodal mutants at E8.5 are defined as Type I, II or III mutants depending on the penetrance of the phenotype.
Results
Specimen 7. A.: embryonic day 8.5 (more )
Note: Also stained for Lefty2 (assay MGI:2159440).
Structure Level Pattern Image Note
TS12: anterior pro-rhombomere floor plate Present Regionally restricted 7A Expression is detected in the left floorplate
TS12: future hindbrain floor plate Present Regionally restricted 7A Expression is detected in the left floorplate
TS12: future midbrain floor plate Present Regionally restricted 7A Expression is detected in the left floorplate
TS12: future prosencephalon floor plate Present Regionally restricted 7A Expression is detected in the left floorplate
TS12: neural tube floor plate Present Regionally restricted 7A Expression is detected in the left floorplate
TS12: posterior pro-rhombomere floor plate Present Regionally restricted 7A Expression is detected in the left floorplate
Specimen 7. B.: embryonic day 8.5; Nodaltm1Mku/Nodaltm1.1Mku (more )
Note: This is a type I mutant; also stained for Lefty2 (assay MGI:2159440).
Structure Level Pattern Image Note
TS12: embryo Absent 7B
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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