About   Help   FAQ
Tg(Mchr1-cre/ERT2)3Nbe - Nervous System
Recombinase Activity Detail
Allele Information
Allele:
Tg(Mchr1-cre/ERT2)3Nbe
transgene insertion 3, Nicolas F Berbari 		Driver: Mchr1
Type: Transgenic (Inducible, Recombinase)
Inducer: tamoxifen
Synonym: Mchr1-CreER
Molecular description: A cre/ERT2 expression sequence was inserted downstream of the Mchr1 promoter on the RP23-202N16 BAC via homologous recombination/BAC recombineering; this replaced nucleotides 4-41 from exon 1 and was designed to disable transcription/translation of the Mchr1 protein from the BAC Mchr1 locus. An frt-flanked kanamycin selection cassette used during homologous recombination/BAC recombineering was removed by transient FLP plasmid injection. The resulting Mchr1-cre/ERT2 BAC transgene was purified for subsequent male pronuclear injection to establish transgenic founder animals. Two lines 2 and 3 were generated with similar expression patterns. .
Find mice (IMSR): Mouse Strains: 0 strains available       Cell Lines: 0 lines available
Additional Tissues: Tg(Mchr1-cre/ERT2)3Nbe activity also observed in:
endocrine system, head
Tissue Information
Nervous System Other recombinase alleles with activity in Nervous System tissues:
Images
Drag images to compare to others or to data in the table below. Drag corners to resize images for more detail. Reset Images
J:266416 Fig. 1 J:266416 Fig. 2 J:266416 Fig. 3
Recombinase Activity
Click heading to re-sort table.
Assays Genotypic Background Result Notes  
References
All for this allele: 1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory