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Gfi1tm2.1(GFP/cre/ERT2)Gan - Head
Recombinase Activity Detail
Allele Information
Allele:
Gfi1tm2.1(GFP/cre/ERT2)Gan
growth factor independent 1 transcription repressor; targeted mutation 2.1, Lin Gan 		Driver: Gfi1
Type: Targeted (Inducible, Recombinase, Reporter)
Inducer: tamoxifen
Synonym: Gfi1-GCE
Molecular description: The coding sequence for P2A-GFP/cre/ERT2 and a downstream FRT-flanked neomycin resistance cassette was inserted in-frame to the C-terminus resulting in bicistronic expression of the endogenous transcript and a tamoxifen-inducible cre recombinase with green fluorescent protein reporter. Breeding to a FLP deleter excised the neo and Immunolabelling of P0 cochlea shows normal nuclear localization of GFI1 and cytoplasmic expression of GFP/cre/ERT2, which migrates to the nucleus after tamoxifen administration. This allele expresses a high level of tamoxifen-inducible cre specific to developing hair cells of the vestibule and cochlea.
Find mice (IMSR): Mouse Strains: 0 strains available       Cell Lines: 0 lines available
Additional Tissues: Gfi1tm2.1(GFP/cre/ERT2)Gan activity also observed in:
sensory organs
Tissue Information
Head Other recombinase alleles with activity in Head tissues:
Images
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J:297267 Fig. 1 J:297267 Fig. 2 J:297267 Fig. 3 J:297267 Fig. 5 J:297267 Fig. 6
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory