P2ry12<em1(icre/ERT2)Tda> - Nervous System Recombinase Activity Detail MGI Mouse

P2ry12^{em1(icre/ERT2)Tda} - Nervous System
Recombinase Activity Detail

Allele Information | Tissue Information | Images | Recombinase Activity | References

Allele Information

Allele:

P2ry12^{em1(icre/ERT2)Tda}
purinergic receptor P2Y, G-protein coupled 12; endonuclease-mediated mutation 1, Thomas D Arnold

Driver: P2ry12
Type: Endonuclease-mediated (Inducible, Recombinase)

Inducer:

tamoxifen

Synonym:

P2ry12-CreER, P2Y12-CreER

Molecular description:

The allele was created using CRISPR/cas9 endonuclease-mediated genome editing to target the stop codon of the P2ry12 locus on chromosome 3. The cas9-RNP complex and a 2A-iCreERT2 dsDNA (encoding a viral 2A oligopeptide that mediates ribosomal skipping and the iCreER fusion gene with ~1.5 kbp homology arms) were injected into the pronucleus of C57BL/6N mouse embryos, followed by adoptive embryo transfer. Founder mice and progeny were screened by PCR, and proper genomic integration was verified by Southern blot and genomic sequencing. Founders were bred to C57BL/6J mice for germline transmission.

Find mice (IMSR):

Mouse Strains: 1 strain available Cell Lines: 0 lines available

Additional Tissues:

P2ry12^{em1(icre/ERT2)Tda} activity also observed in:
alimentary system, cardiovascular system, head, hemolymphoid system, liver & biliary system, respiratory system

Tissue Information

Nervous System

Other recombinase alleles with activity in Nervous System tissues:

7630403G23Rik^{Tg(Th-cre)1Tmd}, Abcg2^{tm1.1(cre/Esr1*)Jvbe}, Acsm4^tm1(cre)Hsak, Adcyap1r1^{tm1.1(cre/GFP)Rpa} ...(more)

Images

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J:291145 Fig. 1

J:291145 Fig. 2

J:291145 Fig. 3

J:339857 Fig. 2

J:339857 Fig. 3

Recombinase Activity

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Assays Genotypic Background Result Notes

References

All for this allele:

3 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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