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Pms2tm2(cre)Lisk - Liver & Biliary System
Recombinase Activity Detail
Allele Information
Allele:
Pms2tm2(cre)Lisk
PMS1 homolog2, mismatch repair system component; targeted mutation 2, Michael Liskay 		Driver: Pms2
Type: Targeted (Recombinase)
Synonym: Pms2cre, Pms2Cre
Molecular description: The SV40 nuclear localization signal (NLS) within the cre sequence was modified to contain an 12-base pair A-T run which preserved the NLS, but added an extra A inducing a +1 bp shift. An IRES was cloned 5' to the cre ATG signal with a neo cassette cloned 3' to the recombinase sequence. The IRES-cre-neo vector was used for homologous recombination in ES cells, resulting in the IRES-cre inserting into the Pms2 locus replacing exon 2. The extra A in the NLS causes the cre to be out of frame (inactive). Because this Pms2 allele is null (thus deficient in DNA mismatch repair), homozygotes will have an increased frequency of -1 bp resulting in cre reversion and thus in cre expression.
Find mice (IMSR): Mouse Strains: 0 strains available       Cell Lines: 0 lines available
Additional Tissues: Pms2tm2(cre)Lisk activity also observed in:
alimentary system, muscle, renal & urinary system
Tissue Information
Liver & Biliary System Other recombinase alleles with activity in Liver & Biliary System tissues:
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 5 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory