About   Help   FAQ
Myf5tm1(cre/Esr1*)Trdo - Limbs
Recombinase Activity Detail
Allele Information
Allele:
Myf5tm1(cre/Esr1*)Trdo
myogenic factor 5; targeted mutation 1, Thomas A Rando 		Driver: Myf5
Type: Targeted (Inducible, Recombinase)
Inducer: tamoxifen
Synonym: Myf5CreER
Molecular description: A targeting vector was designed to place an internal ribosome entry site (IRES) fused to a CreERTM fusion gene (CreERTM; Cre recombinase fused to a G525R mutant form of the mouse estrogen receptor ligand binding domain) downstream of the stop codon in exon 3 of the Myf5 gene. A neomycin resistance marker, flanked by frt sites, was inserted downstream of the IRES-CreERTM cassette. This construct was electroporated into R1 embryonic stem cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were bred with C57BL/6 females.
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Additional Tissues: Myf5tm1(cre/Esr1*)Trdo activity also observed in:
embryo-other, head, mesenchyme, muscle
Tissue Information
Limbs Other recombinase alleles with activity in Limbs tissues:
Images
Drag images to compare to others or to data in the table below. Drag corners to resize images for more detail. Reset Images
J:199482 Fig. 2A J:199482 Fig. 2B J:199482 Fig. 2D J:199482 Fig. 3A J:199482 Fig. 3C J:199482 Fig. 4A J:199482 Fig. 4B J:199482 Fig. 4C
J:199482 Fig. S2B J:199482 Fig. S2C
Recombinase Activity
Click heading to re-sort table.
Assays Genotypic Background Result Notes  
References
All for this allele: 1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory