Rasgrf2<tm1(cre/folA)Hze> - Nervous System Recombinase Activity Detail MGI Mouse

Rasgrf2^{tm1(cre/folA)Hze} - Nervous System
Recombinase Activity Detail

Allele Information | Tissue Information | Images | Recombinase Activity | References

Allele Information

Allele:

Rasgrf2^{tm1(cre/folA)Hze}
RAS protein-specific guanine nucleotide-releasing factor 2; targeted mutation 1, Hongkui Zeng

Driver: Rasgrf2
Type: Targeted (Inducible, Recombinase)

Inducer:

trimethoprim

Synonym:

Rasgrf2-2A-dCre, Rasgrf2-2A-DHFR-Cre, Rasgrf2-2A-ecDHFR^R12Y/Y100I/Cre, Rasgrf2-2A-ecDHFRR12Y/Y100I/Cre, Rasgrf2-2A-hDHFR/Cre

Molecular description:

The targeting vector containied partial Rasgrf2 intron 29-33 sequence containing an frt3 site in intron 33, a partial Rasgrf2 exon 34 sequence up to (but not including) the endogenous stop codon, self-splicing T2A sequence in-frame with the Rasgrf2 coding sequence, a dCre fusion gene in-frame with the Rasgrf2 coding sequence, a bovine growth hormone polyA signal, an AttB site, a PGK-Neo-polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 polyA signal, and an AttP site. The dCre fusion gene (destabilized Cre, hDHFR/Cre or ecDHFR/Cre) is Cre recombinase fused at its N terminus to the first 159 amino acids of the E. coli K-12 strain chromosomal dihydrofolate reductase gene (DHFR or folA) harboring the G67S mutation and modified to include the R12Y/Y100I destabilizing domain mutations. The targeting vector was electroporated into G4 ES cells. Correctly targeted ES cells were injected into recipient blastocysts. When treated with trimethoprim, cre reporter gene activity is observed at high levels in cortical layers 2/3 and other scattered cells of the cortex, hypothalamus, thalamus, and midbrain.

Find mice (IMSR):

Mouse Strains: 1 strain available Cell Lines: 0 lines available

Additional Tissues:

Rasgrf2^{tm1(cre/folA)Hze} activity also observed in:
head

Tissue Information

Nervous System

Other recombinase alleles with activity in Nervous System tissues:

7630403G23Rik^{Tg(Th-cre)1Tmd}, Abcg2^{tm1.1(cre/Esr1*)Jvbe}, Acsm4^tm1(cre)Hsak, Adcyap1r1^{tm1.1(cre/GFP)Rpa} ...(more)

Images

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J:220523 Fig. 6

Recombinase Activity

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Assays Genotypic Background Result Notes

References

All for this allele:

19 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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