Nkx2-1<tm1.1(cre/ERT2)Zjh> - Adipose Tissue Recombinase Activity Detail MGI Mouse

Nkx2-1^{tm1.1(cre/ERT2)Zjh} - Adipose Tissue
Recombinase Activity Detail

Allele Information | Recombinase Activity | References

Allele Information

Allele:

Nkx2-1^{tm1.1(cre/ERT2)Zjh}
NK2 homeobox 1; targeted mutation 1.1, Z Josh Huang

Driver: Nkx2-1
Type: Targeted (Inducible, Recombinase)

Inducer:

tamoxifen

Synonym:

Nkx2.1-CreER

Molecular description:

A targeting vector was designed to insert a CreERT2 fusion gene , an SV40 polyA signal, and an frt-flanked neo cassette into the initiation codon of the NK2 homeobox 1 locus (Nkx2-1). This construct was electroporated into embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with C57BL/6 mice to originate the colony. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background) to remove the neo selection cassette. These Nkx2.1-CreER (or Nkx2.1-CreERT2) mice were subsequently bred to C57BL/6 inbred mice for a few additional generations (and the FLPe transgene was removed).

Collection:

Neuroscience Blueprint cre

Find mice (IMSR):

Mouse Strains: 1 strain available Cell Lines: 0 lines available

Additional Tissues:

Nkx2-1^{tm1.1(cre/ERT2)Zjh} activity also observed in:
alimentary system, endocrine system, head, nervous system, respiratory system

Recombinase Activity

Click heading to re-sort table.

Assays Genotypic Background Result Notes

References

All for this allele:

46 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/23/2024 MGI 6.23