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Nos1tm1.1(cre/ERT2)Zjh - Nervous System
Recombinase Activity Detail
Allele Information
Allele:
Nos1tm1.1(cre/ERT2)Zjh
nitric oxide synthase 1, neuronal; targeted mutation 1.1, Z Josh Huang
Driver: Nos1
Type: Targeted (Inducible, Recombinase)
Inducer: induced by tamoxifen
Synonym: nNOS-CreER-KI, nNOS-CreER-KI/KO, nNOS-CreERT2-KI, Nos1-CreER-KI, Nos1-CreERT2-KI
Molecular description: A targeting vector was designed to insert a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), an SV40 polyA signal, and an frt-flanked neo cassette into the initiation codon of the neuronal nitric oxide synthase 1 locus (Nos1). This construct was electroporated into C57BL/6;129S-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with 129S mice to originate the colony. Mutant mice were bred with Actin-FLPe mice to remove the neo selection cassette and the FLPe transgene was subsequently bred out of the line.
Collection: Neuroscience Blueprint cre
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Additional Tissues: Nos1tm1.1(cre/ERT2)Zjh activity also observed in:
alimentary system, renal and urinary system
Tissue Information
Nervous System Other recombinase alleles with activity in Nervous System tissues:
Images
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Fig. Nos1 Adult Cerebellum
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Fig. Nos1 Adult Cerebral Cortex
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Fig. Nos1 Adult Dorsal Root Ganglion
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Fig. Nos1 Adult Hippocampus
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Fig. Nos1 Adult Hypothalamus
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Fig. Nos1 Adult Medulla
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Fig. Nos1 Adult Midbrain
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Fig. Nos1 Adult Pons
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Fig. Nos1 Adult SC cerv
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Fig. Nos1 Adult SP thoracic
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Fig. Nos1 Adult Ventral Striatum
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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Funding Information
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last database update
08/18/2015
MGI 6.0
The Jackson Laboratory