Ascl1<tm1.1(cre/ERT2)Jejo> - Head Recombinase Activity Detail MGI Mouse

Ascl1^{tm1.1(cre/ERT2)Jejo} - Head
Recombinase Activity Detail

Allele Information | Tissue Information | Recombinase Activity | References

Allele Information

Allele:

Ascl1^{tm1.1(cre/ERT2)Jejo}
achaete-scute family bHLH transcription factor 1; targeted mutation 1.1, Jane Johnson

Driver: Ascl1
Type: Targeted (Inducible, Recombinase)

Inducer:

tamoxifen

Synonym:

Ascl1-CreER, Ascl1^CreERT2

Molecular description:

A targeting vector was designed to replace the entire coding region of the achaete-scute complex homolog 1 (Ascl1 or Mash1) with a CreERT2 fusion protein and a frt-flanked neomycin (neo) selection cassette. The construct was electroporated into 129S/SvEv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric mice were bred to C57BL/6 mice. These mice were then bred to mice containing Flp-recombinase to remove the Frt-flanked sequence. These mice were then backcrossed at least 5 generations to and outbred ICR strain generate a colony of Ascl1-CreERT2 mice.

Find mice (IMSR):

Mouse Strains: 1 strain available Cell Lines: 0 lines available

Additional Tissues:

Ascl1^{tm1.1(cre/ERT2)Jejo} activity also observed in:
nervous system

Tissue Information

Head

Other recombinase alleles with activity in Head tissues:

7630403G23Rik^{Tg(Th-cre)1Tmd}, Abcg2^{tm1.1(cre/Esr1*)Jvbe}, Acan^{tm1(cre/ERT2)Crm}, Acsm4^tm1(cre)Hsak ...(more)

Recombinase Activity

Click heading to re-sort table.

Assays Genotypic Background Result Notes

References

All for this allele:

65 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/23/2024 MGI 6.23