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Erbb4tm1.1(cre/ERT2)Aibs - Nervous System
Recombinase Activity Detail
Allele Information
Allele:
Erbb4tm1.1(cre/ERT2)Aibs
erb-b2 receptor tyrosine kinase 4; targeted mutation 1.1, Allen Institute for Brain Science 		Driver: Erbb4
Type: Targeted (Inducible, Recombinase)
Inducer: tamoxifen
Synonym: Erbb4-2A-CreERT2, Erbb4tm1(cre/ERT2)Aibs
Molecular description: A targeting vector was designed to insert a viral 2A oligopeptide that mediates ribosomal skipping and a CreERT2 fusion gene immediately downstream of the translational STOP codon of the Erbb4 locus (v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)). The targeting vector contained, from 5' to 3', an frt3 site, a viral 2A oligopeptide, a CreERT2 fusion gene, a bovine growth hormone polyA signal, an attB site, a PGK-Neo-polyA cassette, an frt5 site, an RNA splice acceptor, the 3' portion of the hygromycin gene with SV40 polyA signal, and an attP site. This construct was electroporated into G4 ES cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were bred to C57BL/6J mice to generate the Erbb4-2A-CreERT2 mutant colony. These mutant mice were bred to PhiC31-expressing mice (C57BL/6J congenic background) to remove the PGK-Neo-polyA cassette, frt5 site, RNA splice acceptor, and the 3' portion of the hygromycin gene with SV40 polyA signal. Following tamoxifen induction, Erbb4-2A-CreERT2 directs reporter gene expression in scattered interneuron subpopulations of Erbb4-expressing cells in the cortex and hippocampus.
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Additional Tissues: Erbb4tm1.1(cre/ERT2)Aibs activity also observed in:
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Tissue Information
Nervous System Other recombinase alleles with activity in Nervous System tissues:
Images
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Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 14 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
04/16/2024
MGI 6.23 		The Jackson Laboratory