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Tg(Col2a1-rtTA,tetO-cre)22Pjro - Mesenchyme
Recombinase Activity Detail
Allele Information
Allele:
Tg(Col2a1-rtTA,tetO-cre)22Pjro
transgene insertion 22, Peter J Roughley 		Driver: Col2a1
Type: Transgenic (Inducible, Recombinase, Transactivator)
Inducer: doxycycline/tetracycline
Synonym: COL2-rtTA-Cre, Tg(Col2a1-rtTA-tetO-cre)22Pjro
Molecular description: The construct was generated such that a 6 kb fragment of the Col2a1 gene (composed of 3 kb of promoter and 3 kb of intron 1 containing an enhancer sequence) controls the reverse tetracycline transactivator sequence. A tetracycline responsive element heptad sequence (tetO) with a CMV minimal promoter is upstream of the cre recombinase coding sequence in this construct. Cre expression can only occur by transactivation of rtTA by doxycycline. The linearized construct was injected into fertilized oocytes and founder mice were obtained (lines 13, 12, 22, and 24). Cre mRNA expression was detected only in cartilage, not in the brain, liver or skin of embryos exposed to doxycycline.
Find mice (IMSR): Mouse Strains: 0 strains available       Cell Lines: 0 lines available
Additional Tissues: Tg(Col2a1-rtTA,tetO-cre)22Pjro activity also observed in:
limbs, skeletal system, tail
Tissue Information
Mesenchyme Other recombinase alleles with activity in Mesenchyme tissues:
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 6 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
04/16/2024
MGI 6.23 		The Jackson Laboratory