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Tg(Cela1-cre/ERT)1Lgdn - Endocrine System
Recombinase Activity Detail
Allele Information
Allele:
Tg(Cela1-cre/ERT)1Lgdn
transgene insertion 1, Craig Logsdon 		Driver: Cela1
Type: Transgenic (Inducible, Recombinase)
Inducer: tamoxifen
Synonym: BAC-Ela-CreErT, Ela-Cre-ERT2
Molecular description: A mouse BAC (clone RP23-359O19, size 222 kb) carrying the intact elastase I (Cela1) gene was used. The BAC was modified to express CreERT. For recombination-mediated targeting, arms homologous to the sequence of elastase I gene between the last exon and the polyA signal (in lower case) were added to either side of the IRES-Cre-ERT-frt-Kan-frt cassette by PCR. Using homologous recombination, the IRES-Cre-ERT-frt-Kan-frt cassette was inserted into the elastase I gene. The Kan selection marker was then removed by flp-mediated recombination. two BAC-Cela1-CreERT founders were identified by genotyping. Q-PCR based analysis indicated the presence of 2-3 copies of the transgene in BAC-Cela1-CreERT founders.
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Additional Tissues: Tg(Cela1-cre/ERT)1Lgdn activity also observed in:
alimentary system
Images
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J:148748 Fig. 2
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 23 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
04/30/2024
MGI 6.23 		The Jackson Laboratory