targeted mutation 1, Bradford B Lowell
Type: Targeted (knock-in)
An optimized internal ribosome entry sequence (IRES) fused to cre recombinase followed by a neomycin resistance cassette flanked FRT sites was inserted after the ChAT stop codon in BAC (bMQ185k21) using homologous recombination in bacteria. A genomic ChAT fragment with 4kb homology arms on either side of the IRES-Cre insertion site was lifted out from the BAC using reverse recombineering techniques and transformed into ES cells. The neo cassette was excised with Flp recombinase. Cre recombinase activity is reported in all cholinergic neurons. This allele generated from Chattm2(cre)Lowl.