About   Help   FAQ
Tg(Sp7-tTA,tetO-EGFP/cre)1Amc - Limbs
Recombinase Activity Detail
Allele Information
Allele:
Tg(Sp7-tTA,tetO-EGFP/cre)1Amc
transgene insertion 1, Andrew P McMahon 		Driver: Sp7
Type: Transgenic (Cre/Flp)
Inducer: induced by doxycycline
Synonym: Osx-Cre, osterix-Cre, Osx1-GFP::Cre, Osx1-cre, Osx-Cre::GFP
Molecular description: A tTA-regulated GFP::Cre construct, which has the tetracycline-regulated transactivator (tTA) cassette upstream of a tetracycline-responsive element (tetO)-controlled Enhanced Green Fluorescent Protein/cre fusion protein was used to modifiy a bacterial artificial chromosome (BAC) encoding exon 1 of the mouse Sp7 gene (formerly osterix or Osx1). A frt-flanked selection cassette following the tTA-regulated GFP::Cre was removed from correctly targeted bacteria via transient flpe expression. A 5.2 kb portion of the modified BAC (Osx1-GFP::Cre) was used for pronuclear injection into fertilized CD1 embryos.
Find mice (IMSR): Mouse Strains: 1 lines available       Cell Lines: 0 lines available
Additional Tissues: Tg(Sp7-tTA,tetO-EGFP/cre)1Amc activity also observed in:
embryo-other, postnatal-other
Tissue Information
Limbs Other recombinase alleles with activity in Limbs tissues:
Images
Drag images to compare to others or to data in the table below. Drag corners to resize images for more detail. Reset Images
J:114494
Fig. 2
Recombinase Activity
Click heading to resort table.
Assays Genotypic Background Result Notes  
References
All for this allele: 37 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support. 		last database update
05/08/2013
MGI 5.13 		The Jackson Laboratory