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Tg(Sp7-tTA,tetO-EGFP/cre)1Amc - Limbs
Recombinase Activity Detail
Allele Information
Allele:
Tg(Sp7-tTA,tetO-EGFP/cre)1Amc
transgene insertion 1, Andrew P McMahon
Driver: Sp7
Type: Transgenic (Inducible, Inserted expressed sequence, Recombinase)
Inducer: induced by doxycycline
Synonym: osterix-Cre, Osx1-cre, Osx1-GFP::Cre, Osx-Cre, Osx-Cre::GFP, Osx-gfp, The Sp7-Cre-EGFP
Molecular description: A tTA-regulated GFP::Cre construct, which has the tetracycline-regulated transactivator (tTA) cassette upstream of a tetracycline-responsive element (tetO)-controlled Enhanced Green Fluorescent Protein/cre fusion protein was used to modifiy a bacterial artificial chromosome (BAC) encoding exon 1 of the mouse Sp7 gene (formerly osterix or Osx1). A frt-flanked selection cassette following the tTA-regulated GFP::Cre was removed from correctly targeted bacteria via transient flpe expression. A 5.2 kb portion of the modified BAC (Osx1-GFP::Cre) was used for pronuclear injection into fertilized CD1 embryos.
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Additional Tissues: Tg(Sp7-tTA,tetO-EGFP/cre)1Amc activity also observed in:
postnatal-other, skeletal system
Tissue Information
Limbs Other recombinase alleles with activity in Limbs tissues:
Images
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J:114494 Fig. 2
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 62 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
09/09/2014
MGI 5.19
The Jackson Laboratory