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Tg(SLC18A3-cre)AMisa - Nervous System
Recombinase Activity Detail
Allele Information
Allele:
Tg(SLC18A3-cre)AMisa
transgene insertion A, Hidemi Misawa 		Driver: SLC18A3
Type: Transgenic (Recombinase)
Synonym: VAChT-Cre-A, VACht.Cre.Slow
Molecular description: The transgenic construct consisted of an 11.3 kb fragment of the human cholinergic gene locus, containing the first 5 exons of CHAT and the first coding exon of SLC18A3 (located in intron 1 of CHAT). A cassette containing cre followed by IRES-EGFP was inserted at the start codon of the SLC18A3 gene. EGFP expression was undetected. The 11.3 kb fragment included various promoter elements including a distal enhancer, RE1/NRSE, and NGFRE. Founder mice contained approximately 50 copies. Expression of cre recombinase by the human SLC18A3 promoter began at postnatal day 7 and was restricted to a subset of cholinergic neurons in the somatomotor nuclei and medial habenular nucleus. Maximal levels of expression were reached around 8 months ofage, later onset than in the Tg(SLC18a3-cre)KMisa line. Transgenic expression was not observed in visceromotor or other central or peripheral cholinergic neurons.
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Additional Tissues: Tg(SLC18A3-cre)AMisa activity also observed in:
head
Tissue Information
Nervous System Other recombinase alleles with activity in Nervous System tissues:
Images
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J:86160 Fig. 5
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 3 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory