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Hcrttm1.1(flpo)Ksak - Nervous System
Recombinase Activity Detail
Allele Information
Allele:
Hcrttm1.1(flpo)Ksak
hypocretin; targeted mutation 1.1, Kenji Sakimura 		Driver: Hcrt
Type: Targeted (Null/knockout, Recombinase, Reporter)
Synonym: Hcrttm1.1(flpo)Ahky, Orexin-Flp
Molecular description: A targeting construct in which the MC1 promoter-driven diphtheria toxin gene, a 5.44 kb fragment at the 5' site, codon-optimized for mammalian expression Flp recombinase, including a nuclear localization signal cDNA fused to EGFP with the T2A peptide sequence, a Pgk-1 promoter-driven neomycin phosphotransferase gene (neo) flanked by two Dre recognition target (rox) sites was used for homologous recombination in ES cells to insert EGFP-2A-Flp just behind the translational initiation site of the hypocretin gene (Hcrt) in-frame. The neo cassette was removed in the ES cells by Dre recombination. This allele is a null allele confirmed by immunohistochemical studies.
Find mice (IMSR): Mouse Strains: 0 strains available       Cell Lines: 0 lines available
Additional Tissues: Hcrttm1.1(flpo)Ksak activity also observed in:
head
Tissue Information
Nervous System Other recombinase alleles with activity in Nervous System tissues:
Images
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J:277436 Fig. 1
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 4 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
05/14/2024
MGI 6.23 		The Jackson Laboratory