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Vipr2em1.1(cre)Hze - Nervous System
Recombinase Activity Detail
Allele Information
Allele:
Vipr2em1.1(cre)Hze
vasoactive intestinal peptide receptor 2; endonuclease-mediated mutation 1.1, Hongkui Zeng 		Driver: Vipr2
Type: Endonuclease-mediated (Recombinase)
Synonym: Vipr2-IRES2-Cre-D, Vipr2-IRES2-Cre-Delta, Vipr2-IRES2-Cre-Deltaneo
Molecular description: Embryonic stem (ES) cells were used for CRISPR/Cas9 genome engineering. The donor vector sequences contained, from 5' to 3', a partial Vipr2 sequence spanning intron 12 up to and including the endogenous stop codon in exon 13, an internal ribosome entry site 2 sequence (IRES2; allows translation initiation in the middle of an mRNA sequence), a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK/gb2 promoter-Neomycin resistance gene-PGK polyA cassette, a frt5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. In addition, several basepair mutations were introduced into the first 30 nucleotides of the Vipr2 3' UTR within exon 13 to protect the donor vector from CRISPR/Cas9 cleavage. Correctly targeted ES cells (Vipr2-IRES2-Cre-neo genotype) were injected into recipient blastocysts. Chimeric mice were bred to PhiC31-expressing mice to remove the AttB/AttP-flanked sequences (PGK/gb2-Neo-pA::frt5::RNA splice acceptor::3'hygro-pA) and replace it with the recombined AttB/AttP site (AttL).
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Additional Tissues: Vipr2em1.1(cre)Hze activity also observed in:
head
Tissue Information
Nervous System Other recombinase alleles with activity in Nervous System tissues:
Images
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J:328366 Fig. 1 J:328366 Fig. S1
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 17 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
10/29/2024
MGI 6.24 		The Jackson Laboratory