Allele Information
|
Allele:
|
Gad2tm2(cre)Zjh
glutamic acid decarboxylase 2;
targeted mutation 2, Z Josh Huang
|
Driver: Gad2
Type: Targeted
(Recombinase)
|
|
Synonym:
|
Gad2Cre, Gad2-iCre, Gad2-ires-Cre, GAD65-Cre, GAD65-iCre, GAD65-ires-Cre
|
Molecular description:
|
A targeting vector was designed to insert an internal ribosome entry site (IRES), a Cre recombinase sequence, an SV40 polyA signal, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the Gad2 (glutamic acid decarboxylase 2) gene. This construct was electroporated into C57BL6/S129 hybrid embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient tetraploid blastocysts. Chimeric mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background (N10)) to remove the selection cassette. The FLPe transgene was subsequently bred out of the background.
|
Collection:
|
Neuroscience Blueprint cre |
Find mice (IMSR):
|
Mouse Strains:
3
strains available
Cell Lines:
0 lines available
|
Additional Tissues:
|
Gad2tm2(cre)Zjh activity also observed in:
adipose tissue,
alimentary system,
cardiovascular system,
embryo-other,
endocrine system,
head,
hemolymphoid system,
integumental system,
liver & biliary system,
mesenchyme,
muscle,
nervous system,
renal & urinary system,
reproductive system,
respiratory system,
sensory organs,
skeletal system
(less)
|
|