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pH-2III Probe Detail
Nucleotide
Probe/Clone
  • Name
    pH-2III
  • Sequence Type
    cDNA
  • ID
    MGI:12066
  • Region Covered
    hybridizes to exons 2 and 3 of Class I genes
  • Vector Type
    Plasmid
  • Insert Site
    PstI
  • Insert Size
    300bp
  • Note
    Hybridizes to all class I sequences.
Source
  • Species
    mouse, laboratory
  • Strain
    BALB/c
  • Tissue
    lymphocyte
  • Cell Line
    lymphoma cell line RDM-4
Genes
H2 histocompatibility-2, MHC
H2-D1 histocompatibility 2, D region locus 1
Polymorphisms
J:20105 Steinmetz M, et al., Cell. 1981 Apr;24(1):125-34
Notes: A 287 bp PstI fragment was isolated from pH-2III and used in Southern analysis.
Endonuclease Gene Allele Fragments Strains
BamHI H2-D1 d 6.2kb BALB/c
k 5.4kb CBA
J:6934 Shin HS, et al., Cell. 1982 Jul;29(3):969-76
Notes: A 350 bp PstI fragment was isolated from pH-2III and used in Southern analysis. Strains and/or t haplotype designations appear in the allele groupings.
Endonuclease Gene Allele Fragments Strains
BamHI H2 a multiple BALB/c
b multiple AKR, C3H/DiSn, C57BL/6-H2k
c multiple C3.t12/t1, C3.tw12/tw32, C3H-tw2
d multiple B6.K1
e multiple B6.TL
f multiple A/J
g multiple SM/J
h multiple SWR/J
EcoRI H2 a multiple BALB/c, C3H-tw71
b multiple AKR, C3.tw75, C3H/DiSn, C57BL/6-H2k
c multiple C3.t12/t1, C3.tw5, C3.tw12, C3H-tw2, STOCK tw32
d multiple B6.K1, STOCK tw32
e multiple B6.TL, C3.tw12
f multiple A/J, C3.t0
g multiple C3H/DiSn, SM/J
h multiple SWR/J
J:7253 Shin HS, et al., Nature. 1983 Nov 24-30;306(5941):380-3
Notes: t haplotype designations, rather than strain designations appear in the allele groupings.
Endonuclease Gene Allele Fragments Strains
EcoRI H2-D1 a 23kb C3.tw12
b 21kb STOCK tw32
J:7675 Shin HS, et al., Cell. 1984 Dec;39(3 Pt 2):573-8
Notes: Strain and/or t haplotype designations appear in the allele groupings.
Endonuclease Gene Allele Fragments Strains
HindIII H2-D1 a 9.5kb C3.t12, STOCK tw32
b 9.0kb C3H-T/tw5, C3H-T/tw12
c 5.8kb C3.tw5
References
J:11962 Steinmetz M, et al., A pseudogene homologous to mouse transplantation antigens: transplantation antigens are encoded by eight exons that correlate with protein domains. Cell. 1981 Sep;25(3):683-92
J:20105 Steinmetz M, et al., Three cDNA clones encoding mouse transplantation antigens: homology to immunoglobulin genes. Cell. 1981 Apr;24(1):125-34
J:6934 Shin HS, et al., Genetic structure and origin of t haplotypes of mice, analyzed with H-2 cDNA probes. Cell. 1982 Jul;29(3):969-76
J:7253 Shin HS, et al., Inversion in the H-2 complex of t-haplotypes in mice. Nature. 1983 Nov 24-30;306(5941):380-3
J:7675 Shin HS, et al., Gene mapping within the T/t complex of the mouse. IV: The inverted MHC is intermingled with several t-lethal genes. Cell. 1984 Dec;39(3 Pt 2):573-8
J:7933 Lader E, et al., Definitive chromosomal location of the H-2 complex by in situ hybridization to pachytene chromosomes. Immunogenetics. 1985;22(1):49-54
J:8713 Uehara H, et al., The molecular organization of the H-2K region of two t-haplotypes: implications for the evolution of genetic diversity. EMBO J. 1987 Jan;6(1):83-90

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last database update
10/15/2019
MGI 6.14
The Jackson Laboratory