Tg(CAG-mCherry/Plk4)#Reba
Transgene Detail

Summary

• Symbol: Tg(CAG-mCherry/Plk4)#Reba
• Name: transgene insertion, Renata Basto
• MGI ID: MGI:6200620
• Synonyms: Tg(mChPlk4)
• Transgene: Tg(CAG-mCherry/Plk4)#Reba Location: unknown
• Alliance: Tg(CAG-mCherry/Plk4)#Reba page

Transgene origin

• Strain of Origin: C57BL/6N

Transgene description

• Transgene Type: Transgenic (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(CAG-mCherry/Plk4)#Reba expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Plk4 (MGI:101783)	conditional: floxed CAT gene and STOP cassette inserted between promoter and reporter-kinase chimeric gene

• Mutation details: The transgene construct comprises the following elements: the CAG promoter, A loxP site, a chloramphenicol acetyl transferase gene and STOP cassette, a second loxP site, the mCherry red fluorescent gene fused in-frame (via a Gly-Gly-Ser linker) with the mouse polo like kinase 4 cDNA, and SV40 poly(A) signal sequence. The construct was microinjected into the pronuclei of fertilized oocytes. This creates a conditional-ready allele where the inserted chimeric reporter-kinase sequence will only be expressed after cre-mediated excission of the floxed CAT gene. (J:198922)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:198922 Marthiens V, et al., Centrosome amplification causes microcephaly. Nat Cell Biol. 2013 Jul;15(7):731-40
• All: 4 reference(s)