Tg(Tagln-PLA2G2A)AMrj
Transgene Detail

Summary

• Symbol: Tg(Tagln-PLA2G2A)AMrj
• Name: transgene insertion A, Michel Raymondjean
• MGI ID: MGI:5575375
• Synonyms: SM22a-humansPLA2IIA WTA, Tg(Tagln-PLA2G2A)A
• Transgene: Tg(Tagln-PLA2G2A)AMrj Location: unknown
• Alliance: Tg(Tagln-PLA2G2A)AMrj page

Transgene origin

• Strain of Origin: C57BL/6 or DBA/2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Tagln-PLA2G2A)AMrj expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PLA2G2A (5320)

  Pla2g2a

  (MGI:104642)

• Mutation details: The 7.2 kb transgene construct contains the promoter region of the mouse transgelin gene (Tgln; also called Sm22a) extending from -2126 to +4136 bp, including 2126 bp of 5' flanking sequence, the first exon, the first intron and the first 11 bp of the second intron, joined to a cDNA encoding human phospholipase A2, group IIA (platelets, synovial fluid) (PLA2G2A) with a C-terminal V5 epitope tag and polyhistidine (6xHis) sequence. Wild-type line A is estimated by Southern blot analysis to carry more than 50 copies of the transgene. Analysis of liver, lung, skeletal muscle, heart and aorta of transgenic mice for transgene-derived PLA2G2A mRNA by reverse transcription- (RT-) PCR analysis and for the protein by western blot analysis demonstrated expression specifically in tissues enriched in smooth muscle, i.e., aorta, heart and lung. (J:34770, J:82809, J:209787)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

The transgene construct was "injected into the pronuclei of B6D2 fertilized oocytes." (J:82809)

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 3 reference(s)