Tg(UBC-Dclre1c,-GFP)6Jpdv
Transgene Detail

Summary

• Symbol: Tg(UBC-Dclre1c,-GFP)6Jpdv
• Name: transgene insertion 6, Jean-Pierre de Villartay
• MGI ID: MGI:5314243
• Synonyms: ArtT, Tg(UBC-Dclre1c-TAP-iGFP)
• Transgene: Tg(UBC-Dclre1c,-GFP)6Jpdv Location: unknown
• Alliance: Tg(UBC-Dclre1c,-GFP)6Jpdv page

Transgene origin

• Strain of Origin: 129

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(UBC-Dclre1c,-GFP)6Jpdv expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Dclre1c (MGI:2441769)

• Mutation details: This transgene was produced by transfection of single-cell embryos by a self-inactivating lentiviral vector with a truncated 3' LTR into which a loxP site has been introduced; upon integration, the 3' LTR is duplicated at the 5' end of the insertion so that the entire transgene can be excised by Cre recombinase, leaving behind a single loxP-delta-3' LTR. The vector contains the human ubiquitin C promoter upstream of the mouse DNA cross-link repair 1C, PSO2 homolog (S. cerevisiae) (Dclre1c/Artemis) open reading frame joined, in-frame, to a DNA fragment encoding a tandem affinity purification (TAP) tag -- which consists of a calmodulin binding peptide joined via a linker sequence containing a TEV protease cleavage site to two copies of the protein A immunoglobulin G binding domain -- followed by an internal ribosomal entry site (IRES2) and the coding sequence for green fluorescent protein. (J:82809, J:125880, J:181755)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 3 reference(s)