Tg(Lck-ERT2/Tal1)BJrg
Transgene Detail

Summary

• Symbol: Tg(Lck-ERT2/Tal1)BJrg
• Name: transgene insertion B, Joachim R Gothert
• MGI ID: MGI:3761360
• Synonyms: lck-ER^T2-SCL
• Transgene: Tg(Lck-ERT2/Tal1)BJrg Location: unknown
• Alliance: Tg(Lck-ERT2/Tal1)BJrg page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence)
• Inducer: tamoxifen
• Mutation: Insertion
• Mutation details: The expressed sequence is a chimeric cDNA encoding the tamoxifen-responsive mutant human estrogen receptor ligand-binding domain ER^T2 joined, via a 9-aa flexible linker peptide, to mouse T-cell acute lymphocytic leukemia 1 (TAL1/SCL) truncated by 108 bp at its N-terminus. The cDNA was inserted into the p1017 expression vector between the mouse thymocyte-specific, proximal Lck promoter and a human growth hormone minigene beginning within exon 1 and containing splice donor/acceptor sequences and the polyadenylation signal. Thymic mRNA expression was confirmed by RT-PCR analysis. Immunohistochemical analysis of thymic sections with an anti-estrogen receptor antibody demonstrated nuclear translocation of most of the fusion protein following treatment of the mice with OHT, but its retention in the cytoplasm in the absence of OHT. (J:8126, J:79835, J:125689)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Two founder lines, designated A and B, were derived. Line B, which exhibited the strongest induction of an abnormal thymic phenotype, was used for all but preliminary experiments. (J:125689)

References

• Original: J:125689 Gothert JR, et al., NOTCH1 pathway activation is an early hallmark of SCL T-leukemogenesis. Blood. 2007 Nov 15;110(10):3753-62
• All: 4 reference(s)