Tg(ACTB-CCL2)1Onoe
Transgene Detail

Summary

• Symbol: Tg(ACTB-CCL2)1Onoe
• Name: transgene insertion 1, Kazunori Onoe
• MGI ID: MGI:3700797
• Synonyms: hMCP-1 in pHbetaAPr-neo, hMCP-1 Tgm, hMCP-1 transgene
• Transgene: Tg(ACTB-CCL2)1Onoe Location: unknown
• Alliance: Tg(ACTB-CCL2)1Onoe page

Transgene origin

• Strain of Origin: (C57BL/6 x C3H)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(ACTB-CCL2)1Onoe expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CCL2 (6347)

  Ccl2	(MGI:98259)
  Ccl12	(MGI:108224)

• Mutation details: The transgene contains 3 kb of 5' flanking DNA, 78 bp of 5' untranslated sequence and 832 bp of the first intron of the human beta-actin gene driving expression of a full-length cDNA encoding human chemokine (C-C motif) ligand 2 followed by an SV40 polyadenylation signal. RT-PCR analysis demonstrated high levels of human CCL2 mRNA in spleen, thymus, lymph nodes and peritoneal exudate cells and lower levels in liver, testis, brain and kidney of transgenic mice. ELISA showed serum CCL2 to be greatly elevated. (J:118309, J:118310)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:118309 Ato M, et al., Delayed clearance of zymosan-induced granuloma and depressed phagocytosis of macrophages with concomitant up-regulated kinase activities of Src-family in a human monocyte chemoattractant protein-1 transgenic mouse. Immunobiology. 2000 Jan;201(3-4):432-49
• All: 3 reference(s)