Mapping Data

Experiment

• Experiment
  TEXT-Congenic
• Chromosome
  11
• Reference
  J:120319 Wang M, et al., Fine mapping and candidate gene analyses of pulmonary adenoma resistance 1, a major genetic determinant of mouse lung adenoma resistance. Cancer Res. 2007 Mar 15;67(6):2508-16
• ID
  MGI:3711203

Genes

Gene	Assay Type
Par1	resistance/susceptibility
D11Mit70	PCR amplified length variant
Hoxb9	PCR amplified length variant
Itga3	reported elsewhere
Spop	reported elsewhere
Kat7	reported elsewhere
Phospho1	reported elsewhere
Tob1	reported elsewhere
Xylt2	reported elsewhere
Ngfr	reported elsewhere
Phb1	reported elsewhere

Notes

• Experiment
  The Par1 locus on mouse Chromosome 11 was refined to a 2 cM interval between D11Mit70 (54 cM) and Hoxb9 (56 cM) using mouse congenic lines derived from lung tumor resistant donor strain M. spretus and lung tumor susceptible background strain A/J. Lung adenomas were induced via urethane injection in 5 week old animals. Phenotypes were scored 20 weeks later. Congenic animals carrying the Par1 critical region displayed decreased lung adenoma multiplicity compared to control animals from background strain A/J.
  
  The refined Par1 locus contains 49 known genes. SNP and gene expression analysis revealed the following potential candidates. Itga3 (56 cM), Spop (55.6 cM), and Myst2 exhibit increased expression in A/J lungs compared to M. spretus, whereas Phospho1 (55.7 cM) exhibits the opposite expression pattern. Tob1, Xylt2, Ngfr (55.6 cM), Itga3, and Phb (55.6 cM) gene sequences contain nonsynonymous SNPs between A/J and M. spretus strains. Tob1 is of particular interest since mutation of this gene results in multiple tumor types in mice, andoverexpression of human TOB1 has been shown to affect cell growth in vitro.