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Mapping Data
  • Experiment
    TEXT-Genetic Cross
  • Chromosome
  • Reference
    J:54987 Vogel SN, et al., Cutting edge: functional characterization of the effect of the C3H/HeJ defect in mice that lack an Lpsn gene: in vivo evidence for a dominant negative mutation. J Immunol. 1999 May 15;162(10):5666-70
  • ID
  • Experiment
    The authors carried out high resolution mapping of the Tlr4 region on mouse Chromosome 4. The authors used (C3H/Rl x 101/RL)F1 mice carrying deletions around the Tlr4 locus. These mice are maintained in a heterozygotic state with a normal, "balancer" copy of Chromosome 4 to insure viability. The Tyrp1b-11R30M strain carried a deletion which eliminated the Tlr4 region while the Tyrp1b-13R75M starin carried an equivalently large deletion distal to the Tlr4 region. Tyrp1b/Tyrp1b-11R30M mice as well as Tyrp1b/Tyrp1b-13R75M mice were crossed with C3H/HeJ mice which are hyporesponsive to LPS. F1 progeny from these crosses were genotyped for markers that distinguished "balancer" Chromosome 4 from the copy of Chromosome that contained the deletion, as well as C3H/HeJ Chromosome 4 markers. Informative markers used in the analysis were ordered along the chromosome. The order of markers on mouse Chromosome 4 is: D4Mcg10 - D4Nds9 - D4Mcg8 - D4Mit25 - 363o12-T7 - 3f1 - Trim32 - 3b11 - 277i15-T7 - 256f23-T7 -3a4 - 277i15-SP6 - 1h9 - D4Mcg6 - D4Rck137 - 2b9 - D4Mcg28 - D4Mcg27 - D4Mcg21 - D4Mcg16 - D4Mcg24 - D4Mcg26 - D4Mcg15 - D4Mcg18 - D4Mcg25 - D4Mcg22 - Tlr4 - D4Mcg5 - D4Mit178. D4Mcg47, D4Mcg49 and D4Mcg54 are located between 256f23-T7 and D4Mcg6. All of the typed markers including Tlr4 were deleted in the Tyrp1b-11R30M strain but not in the Tyrp1b-13R75M strain.

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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