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Caption (A-C) Primoridal germ cells (PGCs) were visualized by wholemount SSEA1 immunostaining with SSEA1 antibody in E10.25 wild-type (WT), Ror2Y324C/Ror2Y324C (Ror2Y324C), and Wnt5atm1Amc/Wnt5atm1Amc (Wnt5a-/-) embryos. (E-G) Gonadal ridges from E11.5 stained with GCNA, and (I-K) E12.5 male gonads stained with GCNA antibody. The caudal end is down in all images. (D, H, L) Quantification of PGCs in the entire E10.25 embryo, E11.5 and E12.5 gonads from confocal stacks, with individuals denoted as WT (diamonds), Ror2Y324C/Ror2Y324C mutants (triangle), and Wnt5atm1Amc/Wnt5atm1Amc null (circle), and means indicated as bars. Consistent with appearances, a significant reduction of PGCs was observed in Wnt5atm1Amc/Wnt5atm1Amc and from E11.5 onward in Ror2Y324C/Ror2Y324C mutants. We noted no significant difference in the number of PGCs between XX and XY gonads at E12.5 (not shown). Results of the Student's t-test are indicated, * p<0.05, ** p<0.01, *** p<0.001.
Copyright This image is from Laird DJ, PLoS Genet 2011 Dec;7(12):e1002428, an open-access article distributed under the terms of the Creative Commons Attribution License. J:179805
Associated
Alleles
Symbol Name
Ror2Y324C receptor tyrosine kinase-like orphan receptor 2; Tyr324Cys
Wnt5atm1Amc wingless-type MMTV integration site family, member 5A; targeted mutation 1, Andrew P McMahon
Associated
Genotypes
Allelic Composition Genetic Background
Wnt5atm1Amc/Wnt5atm1Amc involves: 129S7/SvEvBrd
Ror2Y324C/Ror2Y324C involves: C57BL/6J

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last database update
08/19/2014
MGI 5.19
The Jackson Laboratory