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Phenotype Image Detail
Caption Histological and ultrastructural analysis reveals apoptosis in Atp2b4tm1Ges/Atp2b4tm1Ges portal vein smooth muscle. Portal veins from wild-type (Pmca4+/+, left panels) and Atp2b4tm1Ges/Atp2b4tm1Ges (Pmca4-/-, right panels) mice of the mixed 129X1/SvJ and Black Swiss backgrounds were examined by light microscopy after staining with hematoxylin and eosin (A-F) or by electron microscopy (G and H). Low magnification (A and B) and higher magnification (C and D) images of fresh tissues revealed no histological abnormalities. After portal veins were hung for 4 hours under experimental conditions used for contractility measurements, nuclei in wild-type smooth muscle (E) appeared normal (boxed section), but the presence of dense nuclei (boxed section) in mutant smooth muscle (F) indicated an increased incidence of cell death. Ultrastructural analysis indicated that wild-type smooth muscle cells were still visible after 4 hours (G), whereas some mutant muscle cells were undergoing apoptosis (H). N, nucleus; arrows, mitochondria in G and H. Note the condensed nucleus, perinuclear and peripheral mitochondria, and loss of organization of the actin-myosin filaments in the mutant cell (H).
Copyright This image is from Okunade GW, J Biol Chem 2004 Aug 6;279(32):33742-50 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:91844
Symbol Name
Atp2b4tm1Ges ATPase, Ca++ transporting, plasma membrane 4; targeted mutation 1, Gary E Shull
Allelic Composition Genetic Background
Atp2b4tm1Ges/Atp2b4tm1Ges involves: 129X1/SvJ * Black Swiss

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