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| Caption | Generation of the Grntm2.1Far allele (targeted allele without Neo). (A) The targeting vector contained an AGG to TGA mutation at nucleotides 1510-1512 of the Grn coding region (black boxes represent coding exons), generating a premature termination codon (star). Gray triangles, loxP sites; black triangles, FRT sites; white box, neomycin resistance cassette (neo); white box, thymadine kinase (TK). Primers used for genotyping PCR and qPCR are denoted with black and blue arrows, respectively. Key restriction enzyme sites are indicated. The position of the Southern blot probe is indicated by the purple bar. | ||||
| Copyright | This image is from Nguyen AD, Proc Natl Acad Sci U S A 2018 Mar 20;115(12):E2849-E2858. Copyright 2018 National Academy of Sciences, U.S.A. J:259651 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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