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| Caption | B, Targeting strategy for generation of the Rhotm1.1Kpal allele.. Top, mouse opsin consists of five exons (E1-5). The opsin enhancer region 2 kb upstream of the opsin gene is indicated by gray line. 2nd row, the targeting vector contained 6.4 kb of 5' and 1.9 kb of 3' homologous sequence with C to A transversion at codon 23 (A inside arrowhead). Vector backbone sequences are indicated by hatched lines, and Beta-lac stands for the Beta-lactamase gene. 3rd row, as a result of homologous recombination, codon 23 in exon 1 was changed to CAC and the flippase recognition target (FRT)-loxP flanked neomycin resistance gene (Neo) was inserted after exon 1. Bottom row, neomycin was removed from targeted locus by cre-loxP recombination. By this knocking-in method, we generated mice with a point mutation at codon 23, which encode the P23H opsin. This mouse lacks the neomycin cassette but has a 174-bp vector backbone insertion in intron between exon 1 and exon 2. | ||||
| Copyright | This image is from Sakami S, J Biol Chem 2011 Mar 25;286(12):10551-67 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:170648 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/30/2024 MGI 6.23 |
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