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Caption Generation of the Ano6tm1.1Naga (TMEM16Gflox) allele. A, schematic representation of wild-type and mutant Ano6 alleles together with the targeting vector. Recognition sites for EcoRI (E), EcoRV (V), KpnI (K), and SmaI (S) in the flanking region of exon 2 (filled boxes) are indicated. In the target vector, a 1.0-kb DNA fragment carrying exon 2 and its flanking region were replaced by a 2.7-kb fragment carrying two loxP sequences (filled arrowheads) and PGK-neo (NeoR) flanked by FRT sequences (gray arrowheads). Diphtheria toxin A fragment (DT-A) driven by the tk promoter was inserted at the 5' site of the vector. In NeoFRTallele, the Ano6 chromosomal gene was replaced by the targeting vector. In Floxed allele, Ano6tm1.1Naga, the FRT-flanked NeoR gene was removed by FLPe recombinase. A deleted allele can be created in which the, the loxP-flanked exon 2 can be deleted by Cre recombinase. Primers used in C are indicated by arrows. Scale bar, 1.0 kb.
Copyright This image is from Suzuki J, J Biol Chem 2013 May 10;288(19):13305-16 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:198369
Symbol Name
Ano6tm1.1Naga anoctamin 6; targeted mutation 1.1, Shigekazu Nagata

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