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| Caption | Generation of the Gnl3tm2.1Rylt (NSflox) and Gnl3tm2.2Rylt (NSnull) allele. (A) The gene-targeting vector contains two loxP sites (black arrowheads) flanking the first two exons, two Frt sites (gray arrowheads) flanking a neomycin-resistance cassette (pgk-neo), and two recombineering arms. Capital letters stand for restriction sites: B, BamHI; P, PstI; H, Hind-3; RV, EcoRV; RI, EcoRI. (B) The correctly targeted ES clones yield a 14.3-kb BamHI-digested fragment from the endogenous allele and a 7.9-kb (or 8.4-kb) fragment from the NSflox-neo (fn) allele when hybridized with the 3' (3'-P) or 5' (5'-P) external probe. The Flp-excised NSflox (f) allele and the germ-line Cre-excised NSnull (n) allele can be verified by BamHI-digested Southern blots hybridized with the 5' external probe. (C) Two correctly targeted ES clones (6H and 7C) were identified by Southern blot and used to establish germ-line transmission of the NSfn allele. (D) NSfn/+ heterozygotes were removed of the pgk-neo cassette to produce NSf/+ mice. A PCR-based genotyping strategy was designed to detect the NSf, NS wild-type (NS+), NSn, and Cre alleles. | ||||||
| Copyright | This image is from Meng L, Proc Natl Acad Sci U S A 2013 Jul 9;110(28):11415-20. Copyright 2013 National Academy of Sciences, U.S.A. J:198698 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/30/2024 MGI 6.23 |
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