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Molecular Image Detail
Caption (A) Schematic representation of the Rev3l locus and gene structure at the 5' end. Vertical lines or boxes represent exons. The shaded areas indicate coding regions. The map is not drawn to scale. (B) Schematic representation of the gene targeting strategy. Bold lines, regions of homology; black boxes, exons; gray boxes, selection markers; tk, thymidine kinase gene; neor, neomycin resistance gene; triangles, loxP sites; B, BamHI sites; E, EcoRI sites. The predicted sizes of DNA fragments detected by Southern blot hybridization with probe A or probe B on EcoRI and BamHI-digested genomic DNA, respectively, as well as the location of the primers polz-1 to polz-6 for the genomic PCRs (short arrows) are indicated. The map is not drawn to scale. The 'targeted locus' depicts the Rev3ltm1Rsky allele and the 'neor deleted locus' depicts the Rev3ltm1.1Rsky allele.
Copyright This image is from Schenten D, J Exp Med 2009 Feb 16;206(2):477-90, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:146453
Symbol Name
Rev3ltm1.1Rsky REV3 like, DNA directed polymerase zeta catalytic subunit; targeted mutation 1.1, Klaus Rajewsky
Rev3ltm1Rsky REV3 like, DNA directed polymerase zeta catalytic subunit; targeted mutation 1, Klaus Rajewsky

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