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Caption (a) The scheme for gene targeting and Cre/FLP-mediated recombination for the Epha4 locus. Only the first three (black vertical bars) of 18 total exons are shown for simplicity. RI, EcoRI; SA, splice acceptor; mCFP, coding sequence for a membrane-targeted cyan fluorescent protein; Neo, neomycin resistance gene; TK, herpes simplex virus thymidine kinase gene; open triangle, Frt site; black triangle, loxP site. Small arrowheads indicate primers used in PCRs to confirm gene targeting or Cre/FLP-mediated recombination or for genotyping. The targeting vector flanked with loxP sites the third exon and two Frt sites flanked a reporter/selection cassette containing a splice acceptor (SA) and an mCFP reporter gene in addition to a Neomycin resistance gene. Homologous recombination results in the targeted allele, Epha4tm1Bzh (EphA4mCFPNeoFlox). The targeted allele was bred to an FLP deleter mouse to excise the SA-mCFP-Neo cassette, giving rise to the conditional allele, Epha4tm1.1Bzh (EphA4Flox) with a floxed exon 3. In a separate mating, the targeted allele was bred to a Cre deleter mouse to excise the floxed exon 3, giving rise to a null allele, Epha4tm1.2Bzh (EphA4mCFPNeoNull) with the knock-in mCFP reporter gene.
Copyright This image is from Herrmann JE, Genesis 2010 Feb;48(2):101-5, and is displayed with the permission of Wiley-Blackwell, who owns the Copyright. J:157159
Symbol Name
Epha4tm1.1Bzh Eph receptor A4; targeted mutation 1.1, Binhai Zheng
Epha4tm1.2Bzh Eph receptor A4; targeted mutation 1.2, Binhai Zheng
Epha4tm1Bzh Eph receptor A4; targeted mutation 1, Binhai Zheng

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