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| Caption | (A) Schematic of the Pold1 domain structure and locations of the modifications with a Pold1 gene targeting method. (B) Structure of the targeting vector and partial restriction map of the murine Pold1 locus before and after homologous recombination (Pold1tm1Uchi; Pold1-) and Cre mediated recombination (Pold1tm1.1Uchi; Pold1exo). Exons are represented by vertical black boxes and introns by intervening horizontal lines. *indicates D400A substitution. The genomic fragment used as a probe for Southern blotting is indicated by the horizontal blue bar. Restriction enzymes: D = DraI; N = NehI. PCR primers P0 and P2 were used to screen for homologous recombinant ES cells. PCR primers P1, P2 and P3 were used for genotyping. | ||||||
| Copyright | This image is from Uchimura A, PLoS ONE 2009;4(1):e4184, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:144992 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/30/2024 MGI 6.23 |
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