Phenotype Image Detail

Image

Caption

Osteogenesis is compromised by the delay in chondrogenic progression and vascular recruitment in Mia3^tm1Lex/Mia3^tm1Lex (Mia3^-/-) bone. (A and B) In contrast to wild-type (WT) embryos, rare Spp1-positive osteoblasts (arrows) are present in the perichondrium of the mutant humerus at 14.5 days post coitum (dpc). (C and D) Mmp9 and PLVAP/MECA-32 are restricted to a few cells within the perichondrium of mutant bones at 14.5 dpc. (E and G) At 16.5 dpc, Mmp9-positive cells remain restricted to the presumptive bone collar perichondrium of mutant embryos. (F and H) A small number of Spp1-positive osteoblasts reside within the hypertrophic zone. (I-N') Masson's trichrome stain (I-L) and hematoxylin/eosin stain (M and N) of WT and mutant humeri at 13.5, 15.5, and 18.5 dpc highlight the onset and progression of the chondrodysplastic phenol-type. Boxed regions (I-N) are enlarged to the right of each set of panels (I'-N'). Bars, 100 um.

Copyright

This image is from Wilson DG, J Cell Biol 2011 May 30;193(5):935-51, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:172737

Associated Alleles

Symbol	Name
Mia3^tm1Lex	MIA SH3 domain ER export factor 3; targeted mutation 1, Lexicon Pharmaceuticals
Mia3^tm1Lex	MIA SH3 domain ER export factor 3; targeted mutation 1, Lexicon Pharmaceuticals

Associated Genotypes

Allelic Composition	Genetic Background
Mia3^tm1Lex/Mia3^tm1Lex	involves: 129S5/SvEvBrd * C57BL/6