Phenotype Image Detail

Image

Caption

Granule cells populate the molecular layer in Bicd2^tm1.1Hgrd/Bicd2^tm1.1Hgrd cerebellar cortex. (a, b) Sagittal sections of P11 and P16 cerebellum stained with H&E. The low magnification in a illustrates the intact folial organization of the vermis of Bicd2^tm1.1Hgrd/Bicd2^tm1.1Hgrd cerebellar cortex. The high magnification in b shows the absence of the internal granule cell layer (igl) and a high density of cells in the molecular layer (ml) in mutant cerebellum. (c) Sagittal sections of P16 cerebellum immunostained with anti-NeuN and anti-calbindin. Note that in the mutant cerebellar cortex the Purkinje cell layer (Pcl, calbindin+ cells) is localized adjacent to the white matter (wm), and that Purkinje cell dendritic arbors extend up to the pial surface. (d-g) Double-labelling confocal immunofluorescence of alpha6 GABAA receptor subunit (alpha6GABAAR) with parvalbumine (Parv) and VGluT1, respectively, in transverse sections of P20 cerebellum. Note the presence of many alpha6GABAAR+ cells (arrow head in e), a moderate amount of Parv+ cells (arrow in e), and abundant VGluT1 labelling (g) in the ml/gl layer of mutant cerebellar cortex. The arrow in f points to a glomerulus containing both alpha6GABAAR+ granule cell dendritic processes and a VGluT1+ mossy fibre ending. Occasionally, double-labelled glomeruli-like structures occur near the Pcl of mutant cerebellar cortex (arrow in g). Optical sections in d-g, 1 um. Scale bars: 200 um (a), 25 um (b, d), 50 um (c).

Copyright

This image is from Jaarsma D, Nat Commun 2014;5():3411, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:210236

Associated Alleles

Symbol	Name
Bicd2^tm1.1Hgrd	BICD cargo adaptor 2; targeted mutation 1.1, Casper Hoogenraad

Associated Genotypes

Allelic Composition	Genetic Background
Bicd2^tm1.1Hgrd/Bicd2^tm1.1Hgrd	involves: C57BL/6