Phenotype Image Detail

Image

Caption

Neuroinflammation and abnormal neuronal architecture in Tg(tetO/Prnp-APP*Swe*Lon)9191Krz/0 Tg(Camk2a-tTA)1Mmay/0 (rTg9191) mice and littermates expressing only tetracycline transactivator (TTA). (A-D) TTA mice showed no apparent reactive gliosis. Brain sections from TTA (A,C) and mutant mice (B,D) at 24 months of age were stained with a monoclonal antibody directed against the microglial marker ionized calcium-binding adaptor molecule 1 (Iba1) (A,B), and an antibody directed against the astrocytic marker glial fibrillary acidic protein (GFAP) (C,D). Congo red was then applied to stain plaques. Representative photomicrographs show staining of the molecular layer of dentate gyrus. No plaques were detected in TTA mice and no activated microglial cells or astrocytes were overtly observed. Scale bars in B and D, 25 um, applies to A-D. (E) TTA mice exhibited no dystrophic neurites. Brain sections were stained with monoclonal antibody SMI-312 to visualize axons (red) and counterstained using thioflavin S. Scale bar, 50 um. All photomicrographs are of brain sections of female mice.

Copyright

This image is from Liu P, PLoS One 2015;10(5):e0126317, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:235351

Associated Alleles

Symbol	Name
Tg(Camk2a-tTA)1Mmay	transgene insertion 1, Mark Mayford
Tg(tetO/Prnp-APP*Swe*Lon)9191Krz	transgene insertion 9191, Kathleen R Zahs

Associated Genotypes

Allelic Composition	Genetic Background
Tg(Camk2a-tTA)1Mmay/0 Tg(tetO/Prnp-APP*Swe*Lon)9191Krz/0	involves: 129S6/SvEvTac * FVB/N