Phenotype Image Detail

Image

Caption

Skeletal muscle groups were visualized in E12.5, E13.5, and E18.5 control and Fat1^tm1.2Fhel/Fat1^tm1.2Fhel (Fat1^{deltaTM/deltaTM}) embryos carrying the Tg(Myl1-lacZ)1Ibdml/0 (MLC3f-2E) transgene, by X-gal staining. Whole mount analysis of skeletal muscles confirms the presence of a reduced cutaneous maximus (CM, red dotted lines) at E12.5, leading to a misshaped CM one day later (E13.5), and the systematic presence of ectopic muscles in the shoulder area (yellow arrow), most frequently inserting between the deltoid and triceps muscles. Flat mounted preparations of the CM dissected from an E18.5 mutant embryo, showing the reduced density as well as randomly oriented multinucleated myofibres (right panels).

Copyright

This image is from Caruso N, PLoS Genet 2013 Jun;9(6):e1003550, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:199157

Associated Alleles

Symbol	Name
Fat1^tm1.2Fhel	FAT atypical cadherin 1; targeted mutation 1.2, Francoise Helmbacher
Tg(Myl1-lacZ)1Ibdml	transgene insertion 1, Developmental Biology Institue of Marseilles-Luminy

Associated Genotypes

Allelic Composition	Genetic Background
Fat1^tm1.2Fhel/Fat1^tm1.2Fhel Tg(Myl1-lacZ)1Ibdml/0	involves: 129S6/SvEvTac * BALB/cJ * C57BL/6J * SJL