Phenotype Image Detail

Image

Caption

Tg(Pax6-HRAS*G12V)2044Ove/0 (Pax6-Ras) transgenic mice show altered lens and corneal development. Heads of E10.5 (A-B'), E11.5 (C-D'), E13.5 (E-F'), E16.5 (G-H') and E18.5 (I-J') nontransgenic (NT) and Pax6-Ras transgenic mice were sectioned and stained with hematoxylin and eosin. Panels A-J' are higher magnifications of boxed regions in A-J, respectively. Asterisks in panels D, D' indicate sectioning artifacts. Arrows in B, B', D and D', point to abnormal clusters of cells in the interior of the lens. Arrow in G' points to the corneal endothelium. Arrows in J point to the vacuoles within the lens. Lens epithelial hyperplasia was seen in the Pax6-Ras transgenic embryos (arrow in F') and Pax6-Ras transgenic lenses failed to detach from the surface ectoderm (D, D'). Eyelids failed to close in Pax6-Ras embryos (H) and the corneal stromal cells were disorganized (F, H, J). K, L. Activated caspase 3 was detected by immunohistochemistry (L, arrows). Antigen-antibody complexes are in red and the nuclei are stained blue with DAPI. Abbreviations; C, corneal epithelium; Cj, conjunctival epithelium; eyl, eyelid; le, lens epithelium; lf, lens fibers; lp, lens pit; nr, neural retina. The scale bar (E') represents 50 um in panels A, B, C, D, K, L, 100 um in panels E, F, 25um in A'-H' and 250 um in panels G-J.

Copyright

This image is from Burgess D, BMC Dev Biol 2010;10():13, an open-access article, licensee BioMed Central Ltd. J:158529

Associated Alleles

Symbol	Name
Tg(Pax6-HRAS*G12V)2044Ove	transgene insertion 2044, Paul A Overbeek

Associated Genotypes

Allelic Composition	Genetic Background
Tg(Pax6-HRAS*G12V)2044Ove/0	involves: FVB/N