Phenotype Image Detail

Image

Caption

Characterization of segmentation in Yap1^tm1Hmc/Yap1^tm1Hmc Six2^{tm1(tTA,tetO-EGFP/cre)Amc}/Six2⁺ (Yap^CM-/-) nephrons. (A-B') Double staining for E-cadherin and Calbindin in RV and SSB. Costaining for Hnf1beta/WT1 (C-D') and Sox9/WT1 (E-F') reveals normal segmentation of hte RV with both proximal and distal segments. Similarly, SSB show normal segmentation. Note the reduced size of the proximal domain in mutant SBB (compare WT1 positive segment in mutants (D',F') to controls (C',E'). This is also apparent in B' and J'. (G-H') Immunofluorescence for E-cadherin and Jag1 reveals no change in specification of the distal RV and the medial segment of the SSB in both genotypes. Note the aberrant morphology (asterisk) of hte site where the connection occurred between the SSB and the UE (B',D',F',H' and J'). (I-J') Immunofluorescence using antibodies to Cytokeratin (UE) and Laminin (BM) shows that fusion occurred before the comma-shaped stages. All staining performed at E15.5. CSB: comma-shaped body; RV: renal vesicle; SSB: S-shaped body. Scale bars represent 25 um. DAPI was used to counterstain nuclei.

Copyright

This image is from Reginensi A, PLoS Genet 2013 Mar;9(3):e1003380, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:195266

Associated Alleles

Symbol	Name
Six2^tm1(tTA,tetO-EGFP/cre)Amc	sine oculis-related homeobox 2; targeted mutation 1, Andrew P McMahon
Yap1^tm1Hmc	yes-associated protein 1; targeted mutation 1, Helen McNeill

Associated Genotypes

Allelic Composition	Genetic Background
Yap1^tm1Hmc/Yap1^tm1Hmc Six2^tm1(tTA,tetO-EGFP/cre)Amc/Six2^+	involves: 129 * 129S6/SvEvTac * C57BL/6J * C57BL/6NCrl