Phenotype Image Detail

Image

Caption

Loss of cap mesenchyme (CM)-derived epithelial structures and abnormal morphogenesis in Yap1^tm1Hmc/Yap1^tm1Hmc Six2^{tm1(tTA,tetO-EGFP/cre)Amc}/Six2⁺ mice. (A-J) Sections of P0 kidneys stained using late nephron markers confirm abnormal nephron formation in mutant kidneys. Glomeruli (Podocin, A,B; Podocin-WT1-Tomato lectin, C-D'). Proximal tubules (LTL, E,F). Henle's loop (Slc12a1, G,H). Distal tubules (Slc12a3, I,J). (K,L) Overview of an E14.5 nephrogenic zone reveals the presence of CM cells (arrows) in both genotypes, but CM-derived epithelial structures (arrowheads) are greatly reduced in mutant when compared to control littermates. (M-N') Higher magnification shows histological morphology defects of mutant S-shaped body (SSB) compared to wild-type controls at E13.5. Scale bars represent 500 um (A,B), 50 um (C-D'), 200 um (E-J), 100 um (K-L).

Copyright

This image is from Reginensi A, PLoS Genet 2013 Mar;9(3):e1003380, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:195266

Associated Alleles

Symbol	Name
Six2^tm1(tTA,tetO-EGFP/cre)Amc	sine oculis-related homeobox 2; targeted mutation 1, Andrew P McMahon
Yap1^tm1Hmc	yes-associated protein 1; targeted mutation 1, Helen McNeill

Associated Genotypes

Allelic Composition	Genetic Background
Yap1^tm1Hmc/Yap1^tm1Hmc Six2^tm1(tTA,tetO-EGFP/cre)Amc/Six2^+	involves: 129 * 129S6/SvEvTac * C57BL/6J * C57BL/6NCrl