Phenotype Image Detail

Image

Caption

Conditional Apc inactivation enhances osteoblast formation and mineral deposition in the developing proximal rib. (A-L) Immunostaining for beta-catenin combined with Alcian blue (AB) staining (A,E), combined von Kossa-Toluidine blue staining (F), hematoxylin/eosin staining (G), gene expression analysis by in situ hybridization with indicated probes for chondrogenic (B,C), osteogenic (D, H-J) and osteoclastogenic differentiation (K) on consecutive transversal sections of the developing proximal rib of a Apc^tm1Rsmi/Apc^tm1Rsmi Tg(Col2a1-cre)1Rsjo/0 (Col2a1-Cre;Apc^15lox/15lox) embryo and control littermate at E14.5 (A-D) and E16.5 (E-K). The double-headed arrows in G indicate the thickness of the rib. (L) Tartrate-resistant acid phosphatase (TRAP) staining of the developing proximal rib of a mutant embryo at E16.5. The boxed region in L is magnified in L' showing multinucleated osteoclasts (arrowheads) staining positive for TRAP. Scale bars: 100 um in A-L; 50 um in L'.

Copyright

This image is from Miclea RL, BMC Dev Biol 2009;9():26, an open-access article, licensee BioMed Central Ltd. J:149225

Associated Alleles

Symbol	Name
Apc^tm1Rsmi	APC, WNT signaling pathway regulator; targeted mutation 1, Ron Smits
Tg(Col2a1-cre)1Rsjo	transgene insertion 1, Randall S Johnson

Associated Genotypes

Allelic Composition	Genetic Background
Apc^tm1Rsmi/Apc^tm1Rsmi Tg(Col2a1-cre)1Rsjo/0	involves: 129P2/OlaHsd * FVB/N