Phenotype Image Detail

Image

Caption

No chondrogenic and osteogenic differentiation in the developing humerus due to lack of functional Apc. (A-B) Immunostaining for beta-catenin combined with Alcian blue (AB) staining (A), combined with von Kossa-Toluidine blue staining (B), and (C-J) gene expression analysis by in situ hybridization with indicated probes for (C-F) chondrocytes, (G-I) osteoblasts and (J) osteoclasts on consecutive transversal sections of the developing humerus of a Apc^tm1Rsmi/Apc^tm1Rsmi Tg(Col2a1-cre)1Rsjo/0 (Col2a1-Cre;Apc^15lox/15lox) embryo and control littermate at E16.5. Beta-catenin-positive spindle-shaped cells organize in clusters and failed to express chondrogenic and osteogenic markers (arrowheads). Beta-catenin-negative cells at the periphery of these clusters expressed early chondrogenic markers only (arrows), probably due to lack of Cre-mediated loss of functional Apc. Scale bars: 100 um.

Copyright

This image is from Miclea RL, BMC Dev Biol 2009;9():26, an open-access article, licensee BioMed Central Ltd. J:149225

Associated Alleles

Symbol	Name
Apc^tm1Rsmi	APC, WNT signaling pathway regulator; targeted mutation 1, Ron Smits
Tg(Col2a1-cre)1Rsjo	transgene insertion 1, Randall S Johnson

Associated Genotypes

Allelic Composition	Genetic Background
Apc^tm1Rsmi/Apc^tm1Rsmi Tg(Col2a1-cre)1Rsjo/0	involves: 129P2/OlaHsd * FVB/N