Phenotype Image Detail

Image

Caption

Localization of primordial germ cells (PGCs). (A and B) Images of an E9.5 wild-type mouse expressing a GFP transgene driven by Pou5f1 (Oct4) promoter to visualize PGCs (A) and Mirc5^tm1Jae/Mirc5^tm1Jae (mir-290-295-/-) embryos expressing this transgene to visualize PGCs (B). (Scale bars, 1 mm.) The numbers in parentheses refer to the number of somite pairs in each embryo. Arrows point to PGCs located near the tail. (C) Number of PGCs, as determined by alkaline phosphatase staining, in the hindgut and mesentery of E9.5 embryos. Black bars indicate the average number of PGCs for each genotype. (D) Number of PGCs, as determined by alkaline phosphatase staining, near the hindlimb and base of tail, in E9.5 embryos. Black bars indicate the average number of PGCs for each genotype.

Copyright

This image is from Medeiros LA, Proc Natl Acad Sci U S A 2011 Aug 23;108(34):14163-8. Copyright 2011 National Academy of Sciences, U.S.A. J:175228

Associated Alleles

Symbol	Name
Mirc5^tm1Jae	microRNA cluster 5, including Mir290a through Mir295; targeted mutation 1, Rudolf Jaenisch

Associated Genotypes

Allelic Composition	Genetic Background
Mirc5^tm1Jae/Mirc5^tm1Jae	involves: 129S4/SvJae * C57BL/6